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Cột Metrosep Amino Acids 1 - 100/4.0 Metrohm 6.4001.410
6.4001.410
Metrohm Thụy Sỹ
12 tháng
HSX: Metrohm - Thụy Sỹ
Ref # pn: 6.4001.410
Separation column for the determination of amino acids by means of UV/VIS detection following PCR with ninhydrin at 120 °C. The Metrosep Amino Acids 1 - 100/4.0 is the standard separation column for amino acids.
Hotline: 0986.817.366 Mr.Việt
Cột phân tích axit amin Metrosep Amino Acids 1 - 100/4.0
HSX: Metrohm - Thụy Sỹ
Order number: 6.4001.410
Separation column for the determination of amino acids by means of UV/VIS detection following PCR with ninhydrin at 120 °C. The Metrosep Amino Acids 1 - 100/4.0 is the standard separation column for amino acids. The column is based on a sulfonated polystyrene-divinylbenzene material. The determination of amino acids is accomplished by means of photometric detection following a post-column reaction with ninhydrin.
The Metrosep Amino Acids 1 - 100/4.0 permits the separation of up to 44 amino acids in research and practice, including all naturally occurring amino acids.
Metrosep Amino Acids 1 - 100/4.0
Chromatogram
Thông số kỹ thuật
Carrier material | Polystyrene-divinylbenzene copolymer with sulfonic acid groups |
---|---|
Column capacity | 2.9 mmol (K+) |
Column dimensions | 100 x 4.0 mm |
Eluents | Citrate phenol eluent (standard eluent) Eluent A: Lithium citrate: 42.6 mmol/L; 17.8 g/2 L Phenol: 10.6 mmol/L; 2.0 g/2 L Hydrochloric acid: pH = 2.8 Eluent B: Lithium citrate: 42.6 mmol/L; 17.8 g/2 L Lithium chloride: 1.0 mol/L; 86.0 g/2 L Phenol: 10.6 mmol/L; 2.0 g/2 L Hydrochloric acid: pH = 4.2 (column temperature 50 °C) PCR reagents Ninhydrin: 0.11 mol/L; 4.0 g/200 mL Hydrindantin: 2.5 mmol/L; 0.16 g/200 mL Dimethyl sulfoxide: 100 mL Lithium acetate buffer (2 mol/L, pH = 5.2 with acetic acid); 100 mL (reactor temperature 120 °C) |
Housing material | Stainless steel |
Max. pressure | 10 MPa |
Maximum flow | 0.5 mL/min |
Organic modifier | 10% acetonitrile 0 - 5% other organic solvents |
Particle size | 5 µm |
pH range | 1–14 |
Standard flow | 0.4 mL/min |
Temperatur range | 30–90 °C |
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